EvoPrinterHD. Instructions: 7
EvoPrinterHD - Tutorial
EvoPrinterHD Identification of Conserved DNA
7. The scorecard for D.willistoni and its ceBLAT alignment are shown below, where the data presented in the scorecard corresponds to that of a ceBLAT alignment spanning 3,570 bp 5' to the fushi tarazu gene. The scorecard broken down into three parts that include the Score, the Start/End of alignment in the input DNA, sequencing gaps and the R/D value. First, the Score reflects the number of bases in the input DNA that align with the test species. Next, Start/End represent the first and last aligning bases in the reference input DNA. Finally, the R/D value provides data for detecting possible rearrangements and/or duplications, where the two numbers shown indicate the bases unique to the either the second or third alignment and the second number indicates those bases that are shared with a higher scoring alignment. In this case, the Score for the second alignment - 307 - matches the number of unique bases found indicating that this alignment has identified a rearrangement in D.willistoni that does not overlap with the first alignment. The thrid alignment also identifies another rearrangement.